Abstract
The prospective effects of Lactobacillus sporogenes (probiotics) and/or praziquantel (PZQ) treatment on some parasitological, histological and molecular aspects in Schistosoma mansoni infected mice were studied. The present data recorded that combination between PZQ (300 mg/Kg one dose 7 weeks post infection) and L. sporogenes (12.5 million spore/mice/week for 8 weeks from the first day of infection) reduced worm and ova count. Also, oogram patterns in liver and intestine recorded that treatment with L. sporogenes alone increase number of dead eggs especially in intestine. Histological observations showed significant (P < 0.05) reduction in the mean values of granuloma diameters in liver and intestine in infected mice groups treated with PZQ and/or L. sporogenes. Single strand breaks (comet assay) showed increase in number of damaged and strong damaged lymphocyte cells in mice infected with S. mansoni and infected treated with PZQ while L. sporogenes administration reduced DNA damage. Flow cytometry also confirmed role of L. sporogenes in reducing significantly DNA damage according to determination of cell cycle analysis apoptosis. It can be concluded that administration of L. sporogenes accompanied with PZQ treatment ameliorates the hepatic and intestinal damage caused by S. mansoni infection.
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Abdel-Salam AM, Ammar N, Abdel-Hamid AZ (2008) Effectiveness of probiotic labneh supplemented with garlic or onion oil against Schistosoma mansoni in infected mice. Int J Dairy Sci 3(2):97–104
Bautista-Garfias CR, Ixta-Rodríguez O, Martínez-Gómez F, Lopez MG, Aguilar-Figueroa BR (2001) Effect of viable or dead Lactobacillus casei organisms administered orally to mice on resistance against Trichinella spiralis infection. Parasite 8(2):S226–S228
Benson A, Pifer R, Behrendt CL, Hooper LV, Yarovinsky F (2009) Gut commensal bacteria direct a protective immune response against Toxoplasma gondii. Cell Host Microbe 6(2):187–196
Boros DL, Tombord R, Warren KS (1977) Introduction of granulomatous and elicitation of cutaneous hypersensitivity by partially purified SEA of Simandin. J Immunol 118:373–376
Botros SS, Bennett JL (2007) Praziquantel resistance. Expert Opin Drug Discov 2(Suppl. 1):S35–S40
Caffrey CR (2007) Chemotherapy of schistosomiasis: present and future. Curr Opin Chem Biol 11:433–439
Chaiworaporn R, Maneerat Y, Rojekittikhun W, Ramasootal P, Janecharut T, Matsuda H, Kitikoon V (2005) Therapeutic effect of subcurative dose Praziquantel on Schistosoma mansoni infected mice and resistance to challenge infection after treatment Southeast Asian. J Trop Med Public Health 36:846–852
Cheever AW (1969) Quantitative comparison of the intensity of Schistosoma mansoni—infections in man and experimental animals. Trans R Soc Trop Med Hyg 63:781–795
Chen L, Rao KV, He YX, Ramaswamy K (2002) Skin-stage schistosomula of Schistosoma mansoni produce an apoptosis-inducing factor that can cause apoptosis of T cells. J Biol Chem 277:34329–34335
Cohen JJ, Al- Rubeai M (1995) Apoptosis-targeted therapies the next big thing in biotechnology? Trends biotechnol 13:281–283
De Moreno de Leblanc A, Perdigón G (2010) The application of probiotic fermented milks in cancer and intestinal inflammation. Proc Nutr Soc 69(3):421–428
De Vecchi E, Drago L (2006) Lactobacillus Sporogenes or Bacillus Coagulans: misidentification or mislabelling? Int J Probiot Prebiot 1:3–10
Dean PN, Jett JH (1974) Mathematical analysis of DNA distributions derived from flow microfluorometry. J Cell Biol 60:523–527
Dell Villar LP, Burguillo FJ, Lopez-Aban J, Muro A (2012) Systematic review and meta-analysis of artemisinin based therapies for the treatment and prevention of schistosomiasis. PLoS One 7(9):e45867
Duvall RH, De witt WB (1969) An improved perfusion technique for recovering adult schistosomes from laboratory animals. Am J Trop Med Hyg 16:438–486
Federica B, David DC, Serena C, Stefano DA (2012) Interactions between parasites and microbial communities in the human gut. Front Cell Infect Microbiol 2:141
Fenwick A, Webster JP (2006) Schistosomiasis: challenges for control, treatment and drug resistance. Curr Opin Infect Dis 19:577–582
Ghoneim HM, Demian SR, Heshmat MG, Ismail NS, El-Sayed LH (2008) Activation-induced apoptosis in peripheral blood mononuclear cells during hepatosplenic Schistosoma mansoni infections. Egypt J Immunol 15(2):63–72
Giboda M, Smith JM (1994) Schistosoma mansoni eggs as a target for praziquantel: efficacy of oral application in mice. J Trop Med Hyg 97(2):98–102
Gnanasekar M, Salunkhe AM, Mallia AK, He YX, Kalyanasundaram M (2009) praziquantel affects the regulatory myosin light chain of Schistosoma mansoni. Antimicrob Agents Chemother 53(3):1054–1060
Harris HF (1990) On the rapid conversion of haematoxylin into haematin staining reaction. J Appl Microsc lab Methods 3:777
Hassab El-Nabi SE (1996) The anti-genotoxic effect of propolies and cloves on human human lymphocytes culture treated with lead nitrate as a heavy metal. J Union Arab Biol Zool 5(A):479–498
Hassab El-Nabi SE, Sallam F (2002) The protective effect of ellagic acid against the mutagenic portal of berelex as one of plant growth regulator hormones using different mutagenic tests. J Egypt Ger Soc Zool 37(C):77–98
Kamel IA, Cheever AW, Elwi AM, Mosimann JE, Danner R (1977) Schistosoma mansoni and S. haematobium infections in Egypt: technique for recovery of worms at necropsy. Am J Trop Med Hyg 26:696–701
Khambualai O, Yamauchi K, Ruttanavut J, Incharoen T, Jun Kashimura J (2010) Effect of sugar cane extract, commercial probiotic and their mixture on growth performance and intestinal histology in broiler chickens. Am J Anim Vet Sci 5(2):132–138
Lin PW, Nasr TR, Berardinelli AJ, Kumar A, Neish AS (2008) The probiotic Lactobacillus GG may augment intestinal host defense by regulating apoptosis and promoting cytoprotective responses in the developing murine gut. Pediatr Res 64(5):511–516
Morsy GH (2009) Parasitological and histo-pathological studies on schistosomiasis mansoni infected mice and treated with praziquatel and/or oltipraz. J Egypt Soc Parasitol 39(2):687–701
Pearce EJ (2005) Priming the immune response by schistosome eggs. Parasite Immunol 27:265–270
Pelicano ERL, Souza PA, Souza HBA, Figueiredo DF, Boiago MM, Carvalho SR, Bordon VF (2005) Intestinal mucosa development in broiler chickens fed natural growth promoters. Braz J Poult Sci 1516-635X:221–229
Pellegrino J, Oliveria CA, Faria J, Cunhan A (1962) New approach to the screening of drugs in experimental S. mansoni in mice. Am J Trop Med Hyg 11:201–215
Peters PA, Warren KS (1969) A rapid method of infecting mice and other laboratory animals with Schistosoma mansoni: subcutaneous injection. J Parasitol 55:558
Singh NP, Mccoy MT, Tice RR, Schneider EL (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191
Son VM, Chang CC, Wu MC, Guu YK, Chiu CH, Cheng W (2009) Dietary administration of the Probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper Epinephelus coioides. Fish Shellfish Immunol 26(5):691–698
Tallima H, El-Ridi R (2007) Praziquantel binds Schistosoma mansoni adult worm actin. Int J Antimicrob Agents 29:570–575
Travers MA, Florent I, Kohl L, Grellier P (2011) Probiotics for the control of parasites: an overview. J Parasitol Res 610769:11
Tribukait B, Moberger G, Zetterberg A (1975) Methodological aspects of rapid-flow cytoflurometry for DNA analysis of human urinary bladder cells. In: Pulse-cytophotometry Part 1, p 50–60
Villena J, Salva S, Agüero G, Alvarez S (2011) Immunomodulatory and protective effect of probiotic Lactobacillus casei against Candida albicans infection in malnourished mice. Microbiol Immunol 55(6):434–445
W H O (2013) Word Health Organization, Geneva. Epidemiological Record No. 8, 88:81–88
Ya T, Zhang Q, Chu F, Merritt J, Bilige M (2008) Immunological evaluation of Lactobacillus casei Zhang: a newly isolated strain from koumiss in Inner Mongolia, China. BMC Immunol 9:68–76
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Mohamed, A.H., Osman, G.Y., Zowail, M.E.M. et al. Effect of Lactobacillus sporogenes (probiotic) on certain parasitological and molecular aspects in Schistosoma mansoni infected mice. J Parasit Dis 40, 823–832 (2016). https://doi.org/10.1007/s12639-014-0586-4
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DOI: https://doi.org/10.1007/s12639-014-0586-4